AN OPTIMISED PIPELINE FOR PARALLEL IMAGE-BASED QUANTIFICATION OF GENE EXPRESSION AND GENOTYPING AFTER IN SITU HYBRIDISATION

An optimised pipeline for parallel image-based quantification of gene expression and genotyping after in situ hybridisation

Advances in genome engineering have resulted in the generation of numerous zebrafish mutant lines.A commonly used method to assess gene expression in the mutants is in situ hybridisation (ISH).Because the embryos can be distinguished by genotype after ISH, comparing gene expression between wild-type and mutant siblings can be done blinded and in pa

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Evaluating the combined effect of sugarcane bagasse ash, metakaolin, and polypropylene fibers in sustainable construction

Abstract The major challenge for the construction industry is to design and produce sustainable construction materials that are efficient in their performance and can be affordable for bovi-shield gold fp 5 l5 construction projects.The objective of this study is to determine the viability of incorporating waste products such as sugarcane bagasse as

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m5C RNA Methylation Regulators Predict Prognosis and Regulate the Immune Microenvironment in Lung Squamous Cell Carcinoma

RNA methylation is a novel epigenetic modification that can be used to evaluate tumor dodge challenger carbon fiber fenders prognosis.However, the underlying mechanisms are unclear.This study aimed to investigate the genetic characteristics of 5-methylcytosine (m5C) and N1-methyladenosine (m1A) regulators in lung squamous cell carcinoma (LUSC) and

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